Open AccessTemporarily Epigenetic Repression in Bergmann Glia Regulates the Migration of Granule Cells
Author(s) -
Chen Shaoxuan,
Zhang Kunkun,
Zhang Boxin,
Jiang Mengyun,
Zhang Xue,
Guo Yi,
Yu Yingying,
Qin Tianyu,
Li Hongda,
Chen Qiang,
Cai Zhiyu,
Luo Site,
Huang Yi,
Hu Jin,
Mo Wei
Publication year - 2021
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.202003164
Subject(s) - transcriptome , microbiology and biotechnology , biology , epigenetics , morphogenesis , neuroscience , downregulation and upregulation , gene expression , gene , genetics
Forming tight interaction with both Purkinje and granule cells (GCs), Bergmann glia (BG) are essential for cerebellar morphogenesis and neuronal homeostasis. However, how BG act in this process is unclear without comprehensive transcriptome landscape of BG. Here, high temporal‐resolution investigation of transcriptomes with FACS‐sorted BG revealed the dynamic expression of genes within given functions and pathways enabled BG to assist neural migration and construct neuron‐glia network. It is found that the peak time of GCs migration (P7‐10) strikingly coincides with the downregulation of extracellular matrix (ECM) related genes, and the disruption of which by Setdb1 ablation at P7‐10 in BG leads to significant migration defect of GCs emphasizing the criticality of Nfix‐Setdb1 mediated H3K9me3 repressive complex for the precise regulation of GCs migration in vivo. Thus, BG's transcriptomic landscapes offer an insight into the mechanism by which BG are in depth integrated in cerebellar neural network.