Open AccessSuper‐Resolution Microscopy: Hypotonic Stress Induces Fast, Reversible Degradation of the Vimentin Cytoskeleton via Intracellular Calcium Release (Adv. Sci. 18/2019)
Author(s) -
Pan Leiting,
Zhang Ping,
Hu Fen,
Yan Rui,
He Manni,
Li Wan,
Xu Jingjun,
Xu Ke
Publication year - 2019
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.201970112
Subject(s) - cytoskeleton , proteolysis , vimentin , calcium in biology , calcium , intracellular , calpain , biophysics , osmotic shock , microbiology and biotechnology , chemistry , degradation (telecommunications) , microscopy , protease , biochemistry , biology , cell , medicine , pathology , enzyme , immunohistochemistry , immunology , telecommunications , gene , computer science , organic chemistry
In article number 1900865, Ke Xu and co‐workers report, through super‐resolution microscopy, an unexpected, fast degradation pathway of the vimentin cytoskeletal system under hypo‐osmotic stress. Together with calcium imaging and biochemical analysis, the study further unveils that this degradation is due to proteolysis by the calcium‐dependent protease calpain, activated by hypotonic stress‐induced calcium release from intracellular stores.