Single‐Cell Cytokine Assays: Multiplexed, Sequential Secretion Analysis of the Same Single Cells Reveals Distinct Effector Response Dynamics Dependent on the Initial Basal State (Adv. Sci. 9/2019) | Zendy

Chen Zhuo | Zendy; Lu Yao | Zendy; Zhang Kerou | Zendy; Xiao Yang | Zendy; Lu Jun | Zendy; Fan Rong | Zendy

Open Access

Single‐Cell Cytokine Assays: Multiplexed, Sequential Secretion Analysis of the Same Single Cells Reveals Distinct Effector Response Dynamics Dependent on the Initial Basal State (Adv. Sci. 9/2019)

Author(s) -

Chen Zhuo,

Lu Yao,

Zhang Kerou,

Xiao Yang,

Lu Jun,

Fan Rong

Publication year - 2019

Publication title -

advanced science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.388

H-Index - 100

ISSN - 2198-3844

DOI - 10.1002/advs.201970055

Subject(s) - secretion , effector , microbiology and biotechnology , population , homogeneous , cell , dynamics (music) , basal (medicine) , biology , chemistry , physics , medicine , biochemistry , endocrinology , environmental health , insulin , thermodynamics , acoustics

In article number 1801361 , Yao Lu, Rong Fan, and co‐workers demonstrate a multiplexed antibody‐barcode‐integrated microtrough array chip, that sequentially measures protein secretions from >5000 single macrophage cells upon activation. The protein secretion dynamics classifies them into two major activation states dependent on the basal state of each cell, showing a cell‐intrinsic heterogeneous response in a phenotypically homogeneous cell population.

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