Open AccessSingle‐Cell Cytokine Assays: Multiplexed, Sequential Secretion Analysis of the Same Single Cells Reveals Distinct Effector Response Dynamics Dependent on the Initial Basal State (Adv. Sci. 9/2019)
Author(s) -
Chen Zhuo,
Lu Yao,
Zhang Kerou,
Xiao Yang,
Lu Jun,
Fan Rong
Publication year - 2019
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.201970055
Subject(s) - secretion , effector , microbiology and biotechnology , population , homogeneous , cell , dynamics (music) , basal (medicine) , biology , chemistry , physics , medicine , biochemistry , endocrinology , environmental health , insulin , thermodynamics , acoustics
In article number 1801361 , Yao Lu, Rong Fan, and co‐workers demonstrate a multiplexed antibody‐barcode‐integrated microtrough array chip, that sequentially measures protein secretions from >5000 single macrophage cells upon activation. The protein secretion dynamics classifies them into two major activation states dependent on the basal state of each cell, showing a cell‐intrinsic heterogeneous response in a phenotypically homogeneous cell population.