Open AccessSmart Hydrogels for the Augmentation of Bone Regeneration by Endogenous Mesenchymal Progenitor Cell Recruitment
Author(s) -
Lienemann Philipp S.,
VallmajoMartin Queralt,
Papageorgiou Panagiota,
Blache Ulrich,
Metzger Stéphanie,
Kiveliö AnnaSofia,
Milleret Vincent,
Sala Ana,
Hoehnel Sylke,
Roch Aline,
Reuten Raphael,
Koch Manuel,
Naveiras Olaia,
Weber Franz E.,
Weber Wilfried,
Lutolf Matthias P.,
Ehrbar Martin
Publication year - 2020
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.201903395
Subject(s) - self healing hydrogels , mesenchymal stem cell , progenitor cell , microbiology and biotechnology , bone morphogenetic protein 2 , in vivo , bone healing , regeneration (biology) , endogeny , bone morphogenetic protein , regenerative medicine , stem cell , biomedical engineering , chemistry , in vitro , biology , medicine , anatomy , biochemistry , organic chemistry , gene
The treatment of bone defects with recombinant bone morphogenetic protein‐2 (BMP‐2) requires high doses precluding broad clinical application. Here, a bioengineering approach is presented that strongly improves low‐dose BMP‐2‐based bone regeneration by mobilizing healing‐associated mesenchymal progenitor cells (MPCs). Smart synthetic hydrogels are used to trap and study endogenous MPCs trafficking to bone defects. Hydrogel‐trapped and prospectively isolated MPCs differentiate into multiple lineages in vitro and form bone in vivo. In vitro screenings reveal that platelet‐derived growth factor BB (PDGF‐BB) strongly recruits prospective MPCs making it a promising candidate for the engineering of hydrogels that enrich endogenous MPCs in vivo. However, PDGF‐BB inhibits BMP‐2‐mediated osteogenesis both in vitro and in vivo. In contrast, smart two‐way dynamic release hydrogels with fast‐release of PDGF‐BB and sustained delivery of BMP‐2 beneficially promote the healing of bone defects. Collectively, it is shown that modulating the dynamics of endogenous progenitor cells in vivo by smart synthetic hydrogels significantly improves bone healing and holds great potential for other advanced applications in regenerative medicine.