Open AccessDandelion‐Like Tailorable Nanoparticles for Tumor Microenvironment Modulation
Author(s) -
Guo Qin,
He Xi,
Li Chao,
He Yongqing,
Peng Yiying,
Zhang Yu,
Lu Yifei,
Chen Xinli,
Zhang Yujie,
Chen Qinjun,
Sun Tao,
Jiang Chen
Publication year - 2019
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.201901430
Subject(s) - tumor microenvironment , in vivo , chemistry , hyaluronic acid , cancer research , macrophage polarization , tumor progression , extracellular matrix , biophysics , in vitro , biochemistry , macrophage , medicine , biology , microbiology and biotechnology , anatomy , tumor cells , gene
Tumor‐associated macrophages (TAMs) constitute over 50% of the number of cells within the tumor, playing a major role in tumor progression and invasion. Remodeling the tumor immune microenvironment by modulating TAM polarization has been emerging as a new and promising therapeutic strategy. However, the high interstitial fluid pressure and dense extracellular matrix lead to insufficient penetration of nanosized therapies. To overcome this dilemma, an acid‐triggered size‐changeable nanoparticle (aptamer/acid sensitive linker crosslinked DGL/zoledronic acid, i.e., Apt@(DGL‐ZA) n NPs) with effective tumor distribution, extravasation, and penetration is designed. Dendrigraft poly‐ L ‐lysines (DGLs) which can induce tumor autophagy as mimics of natural abnormal proteins are crosslinked via a mild‐acid‐responsive linker (1,6‐bis(4‐formylbenzoyloxy) hexane). Long circulation property and tumor penetration are achieved simultaneously by catching DGLs in neutral pH while releasing them in the tumor's pH, like dandelion seeds in midair. The macrophage conditioning agent zoledronic acid (ZA) is loaded on DGLs by the charge attraction. A Tenascin‐C targeting aptamer (GBI‐10) is modified onto (DGL‐ZA) n NPs for a tumor‐homing effect. Apt@(DGL‐ZA) n NPs show both enhanced penetration in in vitro 3D triple negative breast cancer spheroids and in vivo tumor tissues. Effective macrophage regulation, enhanced tumor autophagy, and excellent in vivo antitumor efficacy are achieved, suggesting this tactic as a significant antitumor strategy.