Open AccessHighly Effective and Noninvasive Near‐Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min
Author(s) -
Li Mu,
Li Liqian,
Su Kun,
Liu Xiangmei,
Zhang Tianjin,
Liang Yanqin,
Jing Doudou,
Yang Xianjin,
Zheng Dong,
Cui Zhenduo,
Li Zhaoyang,
Zhu Shengli,
Yeung Kelvin Wai Kwok,
Zheng Yufeng,
Wang Xianbao,
Wu Shuilin
Publication year - 2019
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.201900599
Subject(s) - biofilm , photothermal therapy , photosensitizer , biocompatible material , in vivo , chemistry , staphylococcus aureus , photodynamic therapy , membrane , microbiology and biotechnology , materials science , biophysics , bacteria , nanotechnology , biomedical engineering , photochemistry , biochemistry , biology , medicine , genetics , organic chemistry
Biofilms have been related to the persistence of infections on medical implants, and these cannot be eradicated because of the resistance of biofilm structures. Therefore, a biocompatible phototherapeutic system is developed composed of MoS 2 , IR780 photosensitizer, and arginine–glycine–aspartic acid–cysteine (RGDC) to safely eradicate biofilms on titanium implants within 20 min. The magnetron‐sputtered MoS 2 film possesses excellent photothermal properties, and IR780 can produce reactive oxygen species (ROS) with the irradiation of near‐infrared (NIR, λ = 700–1100 nm) light. Consequently, the combination of photothermal therapy (PTT) and photodynamic therapy (PDT), assisted by glutathione oxidation accelerated by NIR light, can provide synergistic and rapid killing of bacteria, i.e., 98.99 ± 0.42% eradication ratio against a Staphylococcus aureus biofilm in vivo within 20 min, which is much greater than that of PTT or PDT alone. With the assistance of ROS, the permeability of damaged bacterial membranes increases, and the damaged bacterial membranes become more sensitive to heat, thus accelerating the leakage of proteins from the bacteria. In addition, RGDC can provide excellent biosafety and osteoconductivity, which is confirmed by in vivo animal experiments.