Open AccessLabel‐Free Multiphoton Endomicroscopy for Minimally Invasive In Vivo Imaging
Author(s) -
Dilipkumar Ashwathama,
AlShemmary Alaa,
Kreiß Lucas,
Cvecek Kristian,
Carlé Birgitta,
Knieling Ferdinand,
Gonzales Menezes Jean,
Thoma OanaMaria,
Schmidt Michael,
Neurath Markus F.,
Waldner Maximilian,
Friedrich Oliver,
Schürmann Sebastian
Publication year - 2019
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.201801735
Subject(s) - endomicroscopy , autofluorescence , multiphoton fluorescence microscope , in vivo , preclinical imaging , fluorescence lifetime imaging microscopy , colonoscopy , pathology , microscopy , fluorescence , biomedical engineering , inflammation , medicine , fluorescence microscope , materials science , confocal , optics , biology , colorectal cancer , physics , microbiology and biotechnology , cancer
Multiphoton microscopy of cellular autofluorescence and second harmonic generation from collagen facilitates imaging of living cells and tissues without the need for additional fluorescent labels. Here, a compact multiphoton endomicroscope for label‐free in vivo imaging in small animals via side‐viewing needle objectives is presented. Minimal invasive imaging at cellular resolution is performed in colonoscopy of mice without surgical measures and without fluorescent dyes as a contrast agent. The colon mucosa is imaged repeatedly in the same animal in a mouse model of acute intestinal inflammation to study the process of inflammation at the tissue level within a time period of ten days, demonstrating the capabilities of label‐free endomicroscopy for longitudinal studies for the first time.