Open AccessPiezoGRIN : A High‐Pressure Chamber Incorporating GRIN Lenses for High‐Resolution 3D‐Microscopy of living Cells and Tissues
Author(s) -
Schneidereit Dominik,
Schürmann Sebastian,
Friedrich Oliver
Publication year - 2019
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.201801453
Subject(s) - high resolution , high pressure , materials science , microscopy , resolution (logic) , optics , optical microscope , biomedical engineering , composite material , scanning electron microscope , physics , geology , computer science , medicine , remote sensing , artificial intelligence , engineering physics
A high‐pressure optical chamber, PiezoGRIN , that facilitates label‐free 3D high‐resolution live‐cell multiphoton microscopy in thick tissue samples is presented. A set of two Gradient Index (GRIN) rod lenses is integrated into the chamber as an optical guide and allows for the adjustment of the focal plane through the sample providing a field of view volume of 450 × 450 × 500 µm ( x , y , z ). An optical lateral resolution of 0.8 µm is achieved by using two‐photon excitation with 150 fs pulses of a 810 nm titanium–sapphire laser at hydrostatic pressures up to 200 MPa. With the PiezoGRIN setup, it is possible to follow pressure‐induced changes in subcellular structure of unstained vital mouse skeletal muscle tissue up to 200 µm below the tissue surface.