Open AccessLabel‐Free High‐Throughput Leukemia Detection by Holographic Microscopy
Author(s) -
Ugele Matthias,
Weniger Markus,
Stanzel Manfred,
Bassler Michael,
Krause Stefan W.,
Friedrich Oliver,
Hayden Oliver,
Richter Lukas
Publication year - 2018
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.201800761
Subject(s) - myeloid leukemia , cytometry , leukemia , flow cytometry , phase contrast microscopy , pathology , microscopy , digital holographic microscopy , blood smear , medicine , biology , immunology , optics , physics , malaria
Abstract Complete blood count and differentiation of leukocytes (DIFF) belong to the most frequently performed laboratory diagnostic tests. Here, a flow cytometry‐based method for label‐free DIFF of untouched leukocytes by digital holographic microscopy on the rich phase contrast of peripheral leukocyte images, using highly controlled 2D hydrodynamic focusing conditions is reported. Principal component analysis of morphological characteristics of the reconstructed images allows classification of nine leukocyte types, in addition to different types of leukemia and demonstrates disappearance of acute myeloid leukemia cells in remission. To exclude confounding effects, the classification strategy is tested by the analysis of 20 blinded clinical samples. Here, 70% of the specimens are correctly classified with further 20% classifications close to a correct diagnosis. Taken together, the findings indicate a broad clinical applicability of the cytometry method for automated and reagent‐free diagnosis of hematological disorders.