Open Access3D‐Bioprinted Osteoblast‐Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo
Author(s) -
Zhai Xinyun,
Ruan Changshun,
Ma Yufei,
Cheng Delin,
Wu Mingming,
Liu Wenguang,
Zhao Xiaoli,
Pan Haobo,
Lu William Weijia
Publication year - 2018
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.201700550
Subject(s) - 3d bioprinting , viability assay , polyethylene glycol , nanocomposite , ethylene glycol , materials science , peg ratio , osteoblast , chemical engineering , hyaluronic acid , in vivo , self healing hydrogels , tissue engineering , biophysics , chemistry , nanotechnology , in vitro , biomedical engineering , polymer chemistry , biochemistry , organic chemistry , anatomy , microbiology and biotechnology , medicine , finance , engineering , biology , economics
An osteoblast‐laden nanocomposite hydrogel construct, based on polyethylene glycol diacrylate (PEGDA)/laponite XLG nanoclay ([Mg 5.34 Li 0.66 Si 8 O 20 (OH) 4 ]Na 0.66, clay )/hyaluronic acid sodium salt (HA) bio‐inks, is developed by a two‐channel 3D bioprinting method. The novel biodegradable bio‐ink A, comprised of a poly(ethylene glycol) (PEG)–clay nanocomposite crosslinked hydrogel, is used to facilitate 3D‐bioprinting and enables the efficient delivery of oxygen and nutrients to growing cells. HA with encapsulated primary rat osteoblasts (ROBs) is applied as bio‐ink B with a view to improving cell viability, distribution uniformity, and deposition efficiency. The cell‐laden PEG–clay constructs not only encapsulated osteoblasts with more than 95% viability in the short term but also exhibited excellent osteogenic ability in the long term, due to the release of bioactive ions (magnesium ions, Mg 2+ and silicon ions, Si 4+ ), which induces the suitable microenvironment to promote the differentiation of the loaded exogenous ROBs, both in vitro and in vivo. This 3D‐bioprinting method holds much promise for bone tissue regeneration in terms of cell engraftment, survival, and ultimately long‐term function.