Open AccessA Vesicular Stomatitis Virus‐Inspired DNA Nanocomplex for Ovarian Cancer Therapy
Author(s) -
Zhao Wei,
Yang Yuping,
Song Lingling,
Kang Tianyi,
Du Ting,
Wu Yujiao,
Xiong Meimei,
Luo Li,
Long Jianlin,
Men Ke,
Zhang Lan,
Chen Xiaoxin,
Huang Meijuan,
Gou Maling
Publication year - 2018
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.201700263
Subject(s) - vesicular stomatitis virus , ovarian cancer , transfection , cytotoxicity , dna , apoptosis , metastasis , chemistry , cancer research , cancer cell , ethylene glycol , intraperitoneal injection , virus , cancer , biology , virology , medicine , biochemistry , pharmacology , in vitro , gene , organic chemistry
Gene therapy provides a novel method for cancer therapy. This study shows a DNA nanocomplex that is inspired from vesicular stomatitis virus (VSV) for ovarian cancer therapy. This DNA nanocomplex consists of a cationized monomethoxy poly (ethylene glycol)‐poly (d,l‐lactide) (MPEG‐PLA) nanoparticle and a plasmid encoding the matrix protein of vesicular stomatitis virus (VSVMP) that plays a critical role in the VSV‐induced apoptosis of cancer cells. The cationized MPEG‐PLA nanoparticle that is self‐assembled by MPEG‐PLA copolymer and N ‐[1‐(2,3‐dioleoyloxy) propyl]‐ N,N,N ‐trimethylammonium chloride (DOTAP) has low cytotoxicity and high transfection efficiency (>80%). Intraperitoneal administration of the p VSVMP nanocomplex remarkably inhibits the intraperitoneal metastasis of ovarian cancer and does not cause significant systemic toxicity. The apoptosis induction and anti‐angiogenesis are involved in the anticancer mechanism. This work demonstrates a VSV‐inspired DNA nanocomplex that has potential application for the treatment of intraperitoneal metastasis of ovarian cancer.