Open AccessSustained Delivery Growth Factors with Polyethyleneimine‐Modified Nanoparticles Promote Embryonic Stem Cells Differentiation and Liver Regeneration
Author(s) -
Wang Meiyan,
Yang Xiaomei,
Zhang Peng,
Cai Lei,
Yang Xibin,
Chen Youwei,
Jing Yuanya,
Kong Jilie,
Yang Xiaowei,
Sun FangLin
Publication year - 2016
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.201500393
Subject(s) - embryonic stem cell , microbiology and biotechnology , endoderm , stem cell , hepatocyte , regeneration (biology) , regenerative medicine , liver regeneration , transplantation , cellular differentiation , in vivo , chemistry , hepatocyte growth factor , biology , in vitro , medicine , biochemistry , receptor , gene
Stem‐cell‐derived hepatocyte transplantation is considered as a potential method for the therapy of acute and chronic liver failure. However, the low efficiency of differentiation into mature and functional hepatocytes remains a major challenge for clinical applications. By using polyethyleneimine‐modified silica nanoparticles, this study develops a system for sustained delivery of growth factors, leading to induce hepatocyte‐like cells (iHeps) from mouse embryonic stem cells (mESCs) and improve the expression of endoderm and hepatocyte‐specific genes and proteins significantly, thus producing a higher population of functional hepatocytes in vitro. When transplanted into liver‐injured mice after four weeks, mESC‐derived definitive endoderm cells treated with this delivery system show higher integration efficiency into the host liver, differentiated into iHeps in vivo and significantly restored the injured liver. Therefore, these findings reveal the multiple advantages of functionalized nanoparticles to serve as efficient delivery platforms to promote stem cell differentiation in the regenerative medicine.