Open AccessNanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia
Author(s) -
Zhao Xi,
Huang Xiaomeng,
Wang Xinmei,
Wu Yun,
Eisfeld AnnKathrin,
Schwind Sebastian,
GallegoPerez Daniel,
Boukany Pouyan E.,
Marcucci Guido I.,
Lee Ly James
Publication year - 2015
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.201500111
Subject(s) - electroporation , transfection , myeloid leukemia , microrna , cell , intracellular , microbiology and biotechnology , chemistry , rna , computational biology , biology , gene , cancer research , biochemistry
A living cell interrogation platform based on nanochannel electroporation is demonstrated with analysis of RNAs in single cells. This minimally invasive process is based on individual cells and allows both multi‐target analysis and stimulus‐response analysis by sequential deliveries. The unique platform possesses a great potential to the comprehensive and lysis‐free nucleic acid analysis on rare or hard‐to‐transfect cells.