Open AccessBiofunctionalized Nanostructured Zirconia for Biomedical Application: A Smart Approach for Oral Cancer Detection
Author(s) -
Kumar Suveen,
Kumar Saurabh,
Tiwari Sachchidanand,
Srivastava Saurabh,
Srivastava Manish,
Yadav Birendra Kumar,
Kumar Saroj,
Tran Thien Toan,
Dewan Ajay Kumar,
Mulchandani Ashok,
Sharma Jai Gopal,
Maji Sagar,
Malhotra Bansi Dhar
Publication year - 2015
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.201500048
Subject(s) - cubic zirconia , silane , cyclic voltammetry , indium tin oxide , materials science , bovine serum albumin , nuclear chemistry , linear range , chemistry , biosensor , detection limit , chromatography , nanotechnology , electrochemistry , organic chemistry , electrode , ceramic , layer (electronics)
Results of the studies are reported relating to application of the silanized nanostructured zirconia, electrophoretically deposited onto indium tin oxide (ITO) coated glass for covalent immobilization of the monoclonal antibodies (anti‐CYFRA‐21‐1). This biosensing platform has been utilized for a simple, efficient, noninvasive, and label‐free detection of oral cancer via cyclic voltammetry technique. The results of electrochemical response studies conducted on bovine serum albumin (BSA)/anti‐CYFRA‐21‐1/3‐aminopropyl triethoxy silane (APTES)/ZrO 2 /ITO immunoelectrode reveal that this immunoelectrode can be used to measure CYFRA‐21‐1 (oral cancer biomarker) concentration in saliva samples, with a high sensitivity of 2.2 mA mL ng −1 , a linear detection range of 2–16 ng mL −1 , and stability of six weeks. The results of these studies have been validated via enzyme‐linked immunosorbent assay.