Open AccessConjugated Polymer Nanoparticles for Label‐Free and Bioconjugate‐Recognized DNA Sensing in Serum
Author(s) -
Bao Biqing,
Ma Mingfeng,
Zai Huafeng,
Zhang Lei,
Fu Nina,
Huang Wei,
Wang Lianhui
Publication year - 2015
Publication title -
advanced science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.388
H-Index - 100
ISSN - 2198-3844
DOI - 10.1002/advs.201400009
Subject(s) - bioconjugation , conjugated system , polyfluorene , covalent bond , oligonucleotide , nanoparticle , chemistry , dna , amine gas treating , colloidal gold , polymer , combinatorial chemistry , naked eye , photochemistry , biophysics , nanotechnology , detection limit , materials science , organic chemistry , biochemistry , chromatography , biology
Hybridbio/‐synthetic sensory conjugated polymer nanoparticles (CPNs) are developed for selective label‐free detection of target ssDNA in serum. Carboxylic acid‐functionalized anionic polyfluorene nanoparticles are rationally designed as signal amplifying unit to bioconjugate with amine functionalized single stranded oligonucleotides as a receptor. The covalent DNA coating can signicantly improve the photostability of the DNA‐bioconjugated CPNs over a wide range of buffer conditions. Better ssDNA discrimination for the DNA‐bioconjugated CPNs sensor is achieved owing to increased interchain interactions and more efficient exciton transport in nanoparticles. The distinguishable uorescent color for DNA‐bioconjugated CPNs in the presence of target ssDNA allows naked‐eye detection of ssDNA under UV irradiation.