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Gram‐Positive Bacteria Cell Wall Driven Self‐Disassembled Nanovesicles against Methicillin‐Resistant Staphylococcus Aureus
Author(s) -
An HongWei,
Fei Yue,
Yan TongDa,
Lu ChuQi,
Wang ManDi,
Ma Teng,
Zhao BoYan,
Nie JinMei,
Tseng HsianRong,
Li LiLi,
Wang Hao
Publication year - 2020
Publication title -
advanced therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.125
0ISSN - 2366-3987
DOI - 10.1002/adtp.201900217
Subject(s) - cyanine , peptidoglycan , staphylococcus aureus , bacteria , biophysics , bacterial cell structure , nanocarriers , bilayer , chemistry , in vivo , listeria monocytogenes , fluorescence , cell wall , microbiology and biotechnology , drug delivery , biochemistry , biology , membrane , physics , organic chemistry , quantum mechanics , genetics
In the delivery system, high‐performance targeting and local burst‐releasing of antibiotics enable the enhancement of antipathogen efficacy and reduction of drug‐resistance risk. Bacteria cell wall driven self‐disassembled nanovesicles (BSNs) composed of a dense H‐aggregated cyanine bilayer are herein reported. The nanovesicle displays the trigger‐disassembly and turn‐on near infrared (NIR) fluorescence property activated by Gram‐positive bacteria. Mechanistic studies indicate that the interaction between nanovesicles and lipoteinchoic acid within the bacterial cell wall disrupts nanovesicles and the free cyanine molecules insert into the network of peptidoglycan, which brings recovered fluorescence. Meanwhile, owing to the burst release of the oritavancin antibiotic payload inside the nanovesicle, the minimum inhibitory concentration of oritavancin is significantly reduced by fourfold compared to that of the free one. Intriguingly, this smart nanoantibiotic effectively enhances the defense against methicillin‐resistant Staphylococcus aureus both in vitro and in vivo, meanwhile the NIR fluorescence signal enables quantitative bioimaging of the therapuetic outcome.