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Synthetic Vehicles for Encapsulation and Delivery of CRISPR/Cas9 Gene Editing Machinery
Author(s) -
Carboni Valentina,
Maaliki Carine,
Alyami Mram,
Alsaiari Shahad,
Khashab Niveen
Publication year - 2019
Publication title -
advanced therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.125
0ISSN - 2366-3987
DOI - 10.1002/adtp.201800085
Subject(s) - crispr , genome editing , cas9 , nanotechnology , computer science , materials science , biology , gene , genetics
Clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR‐associated 9 (Cas‐9) technology holds tremendous potential as a gene editing tool. Different strategies have been adopted for in vitro and in vivo delivery of CRISPR/Cas9, including both viral and non‐viral. The possibility of tailoring properties of nanosized systems makes the molecular design of self‐assembled non‐viral delivery systems based on organic (lipids and polymers) and hybrid (zeolitic imidazolate frameworks, ZIF and gold nanoparticles) materials of a great interest in CRISPR/Cas9 delivery. This review highlights the progress and challenges of organic and hybrid CRISPR/Cas9 delivery vehicles.