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High‐Level Production of Phenylacetaldehyde using Fusion‐Tagged Styrene Oxide Isomerase
Author(s) -
Choo Joel P. S.,
Kammerer Richard A.,
Li Xiaodan,
Li Zhi
Publication year - 2021
Publication title -
advanced synthesis and catalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.541
H-Index - 155
eISSN - 1615-4169
pISSN - 1615-4150
DOI - 10.1002/adsc.202001500
Subject(s) - chemistry , styrene oxide , phenylacetaldehyde , styrene , isomerization , substrate (aquarium) , yield (engineering) , catalysis , organic chemistry , copolymer , polymer , oceanography , materials science , metallurgy , geology
An order‐of‐magnitude improvement in the production of phenylacetaldehyde to 3.37 M (405 g L −1 ) from the enzymatic isomerisation of styrene oxide was achieved. A small ubiquitin‐related modifier (SUMO) tag increases the productivity of the whole‐cell biocatalytic system by enhancing the expression of active membrane‐bound styrene oxide isomerase (SOI) while retaining enzyme catalytic efficiency and broad natural substrate scope. The isomerisation was performed by using Escherichia coli expressing SUMO‐tagged SOI in an organic‐aqueous biphasic system to yield 96% of phenylacetaldehyde.