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Application of Transaminases in a Disperse System for the Bioamination of Hydrophobic Substrates
Author(s) -
Fiorati Andrea,
Berglund Per,
Humble Maria S.,
Tessaro Davide
Publication year - 2020
Publication title -
advanced synthesis and catalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.541
H-Index - 155
eISSN - 1615-4169
pISSN - 1615-4150
DOI - 10.1002/adsc.201901434
Subject(s) - chemistry , reductive amination , amination , stereoselectivity , amine gas treating , biocatalysis , solvent , pulmonary surfactant , organic chemistry , combinatorial chemistry , solvent polarity , ionic bonding , green chemistry , ionic liquid , chemical engineering , catalysis , biochemistry , ion , engineering
The challenging bioamination of hydrophobic substrates has been attained through the employment of a disperse system consisting of a combination of a low polarity solvent (e. g. isooctane or methyl‐ tert ‐butylether), a non‐ionic surfactant and a minimal amount of water. In these conditions, amine transaminases (ATA) were shown to efficiently carry out the reductive amination of variously substituted cyclohexanones, providing good conversions often coupled with a superior stereoselectivity if compared with the corresponding chemical reductive amination. An array of synthetically useful 4‐substituted aminocyclohexanes was consequentially synthesized through biocatalysis, analyzed and stereochemically characterized.