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Donor Amine Salt‐Based Continuous in situ‐ Product Crystallization in Amine Transaminase‐Catalyzed Reactions
Author(s) -
Hülsewede Dennis,
Dohm JanNiklas,
von Langermann Jan
Publication year - 2019
Publication title -
advanced synthesis and catalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.541
H-Index - 155
eISSN - 1615-4169
pISSN - 1615-4150
DOI - 10.1002/adsc.201900217
Subject(s) - isopropylamine , chemistry , amine gas treating , crystallization , catalysis , salt (chemistry) , transaminase , solubility , dissolution , organic chemistry , inorganic chemistry , enzyme
The unfavorable reaction equilibrium of transaminase‐catalyzed reactions is a major challenge for the efficient biocatalytic synthesis of chiral amines. In this study the synthetic utilization of a salt‐based, continuous in situ ‐product crystallization is described to overcome the thermodynamic limit in amine transaminase‐reactions using only the commonly used amine donor isopropylamine. The simultaneous dissolution of isopropylammonium 3,3‐diphenylpropionate (donor salt) in combination with the crystallization of the product salt facilitates a thermodynamic shift of the continuous amine transaminase‐catalyzed reaction. The main process necessity is a lower product salt solubility in comparison to the applied donor salt. This concept facilitates a stoichiometric use of isopropylamine in combination with a significantly lowered concentration of the amines in solution.