Premium
Designer Microorganisms for Optimized Redox Cascade Reactions – Challenges and Future Perspectives
Author(s) -
Bayer Thomas,
Milker Sofia,
Wiesinger Thomas,
Rudroff Florian,
Mihovilovic Marko. D.
Publication year - 2015
Publication title -
advanced synthesis and catalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.541
H-Index - 155
eISSN - 1615-4169
pISSN - 1615-4150
DOI - 10.1002/adsc.201500202
Subject(s) - synthetic biology , metabolic engineering , biochemical engineering , redox , biocatalysis , chemistry , cascade , nanotechnology , computational biology , metabolic pathway , biochemistry , biology , enzyme , reaction mechanism , organic chemistry , engineering , catalysis , materials science , chromatography
An immense number of chemical reactions are carried out simultaneously in living cells. Nature’s optimization approach encompasses the assembly of reactions in cascades and to embed them in finely tuned metabolic networks. With the vast progress in the field of biocatalysis, man‐made cascades, especially redox cascades, have reached a degree of complexity that needs tools for improved control and optimization. Combined strategies from biocatalysis, metabolic engineering and synthetic biology lead to the establishment of artificial metabolic pathways with minimized interference with the cellular host environment. This review will focus on genetic and metabolic engineering tools for the assembly and introduction of de novo redox pathways into the host Escherichia coli and will present state of the art redox cascades performed by tailor‐made microbial cell factories.