Premium
Deracemization of Amino Acids by Coupling Transaminases of Opposite Stereoselectivity
Author(s) -
Park EulSoo,
Shin JongShik
Publication year - 2014
Publication title -
advanced synthesis and catalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.541
H-Index - 155
eISSN - 1615-4169
pISSN - 1615-4150
DOI - 10.1002/adsc.201400185
Subject(s) - chemistry , stereoselectivity , deamination , isopropylamine , oxidative deamination , transamination , oxidase test , enantiomer , hydrogen peroxide , amino acid , enantiomeric excess , combinatorial chemistry , organic chemistry , stereochemistry , enantioselective synthesis , catalysis , enzyme , biochemistry
Biocatalytic deracemization of amino acids without relying on oxidase‐based deamination of an unwanted enantiomer was demonstrated by coupling α‐ and ω‐transaminases displaying opposite stereoselectivity. This strategy employs isopropylamine and a keto acid as cosubstrates and is free of generation of hydrogen peroxide which is troublesome in the conventional oxidase‐based methods.