One‐Pot Synthesis of Spirooxazino Derivatives via Enzyme‐ Initiated Multicomponent Reactions

A novel enzyme‐initiated multicomponent reaction from readily available aldehyde, nitrostyrene, cyclohexanone and acetamide substrates was discovered, enabling the facile construction of six new CC/N bonds and two rings in single step, one‐pot operation, for the synthesis of spirooxazino derivatives in moderate to high yields. Several methods such as isotope labelling and enzyme mutation were used to probe the possible mechanism of this complex synthesis.