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Enzyme Immobilization: The Quest for Optimum Performance
Author(s) -
Sheldon Roger A.
Publication year - 2007
Publication title -
advanced synthesis and catalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.541
H-Index - 155
eISSN - 1615-4169
pISSN - 1615-4150
DOI - 10.1002/adsc.200700082
Subject(s) - chemistry , self healing hydrogels , immobilized enzyme , enzyme , mesoporous material , polymer , entrapment , aqueous solution , chemical engineering , nanotechnology , combinatorial chemistry , organic chemistry , catalysis , materials science , medicine , surgery , engineering
Immobilization is often the key to optimizing the operational performance of an enzyme in industrial processes, particularly for use in non‐aqueous media. Different methods for the immobilization of enzymes are critically reviewed. The methods are divided into three main categories, viz. (i) binding to a prefabricated support (carrier), (ii) entrapment in organic or inorganic polymer matrices, and (iii) cross‐linking of enzyme molecules. Emphasis is placed on relatively recent developments, such as the use of novel supports, e.g., mesoporous silicas, hydrogels, and smart polymers, novel entrapment methods and cross‐linked enzyme aggregates (CLEAs).