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Cross‐Linked Aggregates of the Hydroxynitrile Lyase from Manihot esculenta : Highly Active and Robust Biocatalysts
Author(s) -
Chmura Andrzej,
van der Kraan Geert M.,
Kielar Filip,
van Langen Luuk M.,
van Rantwijk Fred,
Sheldon Roger A.
Publication year - 2006
Publication title -
advanced synthesis and catalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.541
H-Index - 155
eISSN - 1615-4169
pISSN - 1615-4150
DOI - 10.1002/adsc.200606140
Subject(s) - chemistry , hydrocyanation , manihot esculenta , limiting , lyase , precipitation , enzyme , organic chemistry , botany , catalysis , mechanical engineering , physics , meteorology , engineering , biology
The precipitation and cross‐linking into CLEAs of the hydroxynitrile lyase (E.C. 4.1.2.10) from Manihot esculenta was investigated and an optimized procedure, which involved precipitation with (NH 4 ) 2 SO 4 , was developed. It was found that a fast (photometric) assay severely underestimated the activity recovery in the CLEA due to rate‐limiting diffusion, whereas in a synthetic assay the activity recovery was up to 93 % of the starting activity. The CLEA was applied in the hydrocyanation of various aldehydes and ketones in microaqueous medium, conditions that rendered the non‐enzymatic background reaction insignificant, even with unreactive substrates.