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Bioreactors Based on Monolith‐Supported Ionic Liquid Phase for Enzyme Catalysis in Supercritical Carbon Dioxide
Author(s) -
Lozano Pedro,
GarcíaVerdugo Eduardo,
Piamtongkam Rungtiwa,
Karbass Naima,
De Diego Teresa,
Burguete M. Isabel,
Luis Santiago V.,
Iborra José L.
Publication year - 2007
Publication title -
advanced synthesis and catalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.541
H-Index - 155
eISSN - 1615-4169
pISSN - 1615-4150
DOI - 10.1002/adsc.200600554
Subject(s) - chemistry , candida antarctica , monolith , supercritical fluid , ionic liquid , supercritical carbon dioxide , bioreactor , microreactor , chromatography , styrene , catalysis , chemical engineering , divinylbenzene , organic chemistry , immobilized enzyme , lipase , polymer , copolymer , enzyme , engineering
Abstract Bioreactors with covalently supported ionic liquid phases (SILP) were prepared as polymeric monoliths based on styrene–divinylbenzene or 2‐hydroxyethyl methacrylate–ethylene dimethacrylate, and with imidazolium units loadings ranging from 54.7 to 39.8 % wt IL per gram of polymer. The SILPs were able to absorb Candida antarctica lipase B (CALB), leading to highly efficient and robust heterogeneous biocatalysts. The bioreactors were prepared as macroporous monolithic mini‐flow systems and tested for the continuous flow synthesis of citronellyl propionate in supercritical carbon dioxide (scCO 2 ) by transesterification. The catalytic activity of these mini‐flow‐bioreactors remained practically unchanged for seven operational cycles of 5 h each in different supercritical conditions. The best results were obtained when the most hydrophobic monolith, M‐SILP‐ 8 ‐CALB, was assayed at 80 °C and 10 M Pa, reaching a total turnover number (TON) of 35.8×10 4 mol product/mol enzyme. The results substantially exceeded those obtained for packed‐bed reactors with supported silica‐CALB‐Si‐4 catalyst under the same experimental conditions.