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A Thermostable Aldolase for the Synthesis of 3‐Deoxy‐2‐ulosonic Acids
Author(s) -
Lamble Henry J.,
Royer Sylvain F.,
Hough David W.,
Danson Michael J.,
Taylor Garry L.,
Bull Steven D.
Publication year - 2007
Publication title -
advanced synthesis and catalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.541
H-Index - 155
eISSN - 1615-4169
pISSN - 1615-4150
DOI - 10.1002/adsc.200600520
Subject(s) - aldolase a , chemistry , aldol reaction , stereochemistry , biocatalysis , combinatorial chemistry , biochemistry , enzyme , catalysis , reaction mechanism
A stereochemically promiscuous 2‐keto‐3‐deoxygluconate aldolase has been used as an efficient biocatalyst to catalyse the aldol reaction of pyruvate with C 3 ‐ and C 4 ‐aldoses to afford syn ‐ and anti ‐3‐deoxy‐2‐ulosonic acids in poor to good de . A continuous flow bioreactor containing immobilised aldolase has been developed that enables gram quantities of C 6 ‐ and C 7 ‐3‐deoxyhept‐2‐ulosonic acids to be produced in an efficient manner.