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Synthesis of GDP‐Fucose on a Soluble Support: A Donor Substrate for the Fucosyltransferases
Author(s) -
Daniellou Richard,
Le Narvor Christine
Publication year - 2005
Publication title -
advanced synthesis and catalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.541
H-Index - 155
eISSN - 1615-4169
pISSN - 1615-4150
DOI - 10.1002/adsc.200505170
Subject(s) - fucose , chemistry , ethylene glycol , substrate (aquarium) , galactose , acceptor , polymer chemistry , organic chemistry , biochemistry , combinatorial chemistry , oceanography , physics , condensed matter physics , geology
The synthesis of GDP‐fucose on a poly(ethylene glycol)‐based soluble support is described. Our strategy relies on the attachment of the 6‐deoxy‐6‐thio‐ L ‐galactose onto the polymer via a thioether linkage , and its conversion to GDP‐fucose using established methods. We thus obtained GDP‐fucose bound to the polymer that was tested as an acceptor for the recombinant fucosyltranferase FucT‐III.