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Novel Hydrolases from Thermophilic Filamentous Fungi for Enantiomerically and Enantiotopically Selective Biotransformations
Author(s) -
Bódai Viktória,
Peredi Réka,
Bálint József,
Egri Gabriella,
Novák Lajos,
Szakacs György,
Poppe László
Publication year - 2003
Publication title -
advanced synthesis and catalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.541
H-Index - 155
eISSN - 1615-4169
pISSN - 1615-4150
DOI - 10.1002/adsc.200303027
Subject(s) - chemistry , lipase , thermophile , acetone , enantiomer , organic chemistry , enzyme , kinetic resolution , selectivity , vinyl acetate , carboxylesterase , enantioselective synthesis , catalysis , polymer , copolymer
Fourteen thermophilic filamentous fungi were cultivated on two shake flask media and the supernatants were assayed for lipase/carboxylesterase activities using olive‐oil, p ‐nitrophenyl palmitate and p ‐nitrophenyl butyrate as substrates. The crude enzyme powders (acetone precipitated supernatants) were tested as biocatalysts in organic solvents. Kinetic resolution of racemic 1‐phenylethanol ( rac ‐ 1 ) and desymmetrisation of 2‐acyloxypropan‐1,3‐diols ( 3a, b ) by acetylation with vinyl acetate were chosen for testing the biocatalytic abilities of these preparations. The tested biocatalysts proved to be comparable to the commercially available enzymes with respect to the degree of enantiomer selectivity, whereas they exhibited a wider range of enantiotopic selectivity than the most common commercial enzymes.