Penicillin Acylase Catalysed Synthesis of Ampicillin in Hydrophilic Organic Solvents | Zendy

van Langen Luuk M. | Zendy; Oosthoek Natasja H. P. | Zendy; van Rantwijk Fred | Zendy; Sheldon Roger A. | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Penicillin Acylase Catalysed Synthesis of Ampicillin in Hydrophilic Organic Solvents

Author(s) -

van Langen Luuk M.,

Oosthoek Natasja H. P.,

van Rantwijk Fred,

Sheldon Roger A.

Publication year - 2003

Publication title -

advanced synthesis and catalysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.541

H-Index - 155

eISSN - 1615-4169

pISSN - 1615-4150

DOI - 10.1002/adsc.200303023

Subject(s) - chemistry , penicillin amidase , acetonitrile , ampicillin , yield (engineering) , penicillin , solvent , alcaligenes faecalis , organic chemistry , immobilized enzyme , enzyme , bacteria , antibiotics , biochemistry , materials science , biology , metallurgy , genetics

Penicillin acylase (EC 3.5.1.11) from Alcaligenes faecalis , immobilised as a cross‐linked enzyme aggregate (CLEA), catalysed the synthesis of ampicillin in water‐miscible organic solvents at low water concentrations. Below 4% water (v/v) no reaction was observed, showing the crucial role of water in maintaining the activity of penicillin acylase. The initial value of S/H was strongly affected by the nature of the solvent, but the effect of the water content was slight in the studied range of 4 to 15%. A reaction in acetonitrile containing 8% water afforded ampicillin in up to 86% yield.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research