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High Throughput Discovery of Alcohol Dehydrogenases for Industrial Biocatalysis
Author(s) -
Ravot Gilles,
Wahler Denis,
FavreBulle Olivier,
Cilia Valérie,
Lefevre Fabrice
Publication year - 2003
Publication title -
advanced synthesis and catalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.541
H-Index - 155
eISSN - 1615-4169
pISSN - 1615-4150
DOI - 10.1002/adsc.200303019
Subject(s) - biocatalysis , chemistry , toolbox , computational biology , drug discovery , biochemical engineering , substrate (aquarium) , combinatorial chemistry , synthetic biology , biochemistry , biology , computer science , catalysis , reaction mechanism , engineering , ecology , programming language
To speed up the discovery of novel biocatalysts for industrial synthesis, an efficient screening approach has been set up. The huge potential of natural biodiversity has been taken advantage of in a discovery protocol using bioinformatics analysis, polymerase chain reaction (PCR) techniques and direct in vitro expression of enzymes. This approach led to the direct characterization of 19 novel thermoactive alcohol dehydrogenases with a broad substrate range, that set up a useful enzymatic “toolbox” for fine chemistry synthesis.