Premium
Discovery of Targeted Material Binding Microorganisms Using a Centrifugal Microfluidic Platform
Author(s) -
Han SongI,
Sarkes Deborah A.,
Jahnke Justin P.,
Hurley Margaret M.,
Ugaz Victor M.,
Renberg Rebecca L.,
Sumner James J.,
StratisCullum Dimitra N.,
Han Arum
Publication year - 2021
Publication title -
advanced materials technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.184
H-Index - 42
ISSN - 2365-709X
DOI - 10.1002/admt.202100282
Subject(s) - microfluidics , biopanning , materials science , nanotechnology , centrifugal force , drug discovery , peptide library , biological system , chemistry , bioinformatics , peptide sequence , rotational speed , biology , mechanical engineering , biochemistry , engineering , gene
Discovery of unique peptides that specifically bind to target materials of interest is commonly achieved through biopanning‐based screening of cell libraries having high diversity. However, conventional methods have limitations in the generation of controllable shear stress applied in order to effectively remove weak and unbound cells from a target material. Here, a centrifugal force‐based microfluidic biopanning platform is presented. Using this microfluidic platform, a wide range of shear stress conditions can be generated by simply adjusting the rotation speed of the platform, which provides a precisely controllable wash step. The enhanced circularly permuted outer membrane protein X (eCPX) 3.0 bacterial peptide display library (≈10 11 number of cells) is screened, and many isolates that show strong affinity towards gold only, ITO only, or both are successfully obtained through a multiround iterative sorting process. Amino acid contents of the resulting isolates are analyzed to provide a better understanding of the distinctive attributes of these peptides and their specific material‐binding capabilities. This platform is simple and easy to use and can be readily applied to screen microbial libraries against virtually any material surface of interest, which can greatly accelerate the discovery of peptide‐driven biomaterials and the development of hybrid organic–inorganic materials.