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Design Artificial Stem Cell Nests for Stem Cell Niche in a Microfluidic Petri Dish Programmed by a Cell Phone
Author(s) -
Peng Xing Yue Larry,
Guo Yaxin,
Peng Linghan,
Liu Juan
Publication year - 2021
Publication title -
advanced materials technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.184
H-Index - 42
ISSN - 2365-709X
DOI - 10.1002/admt.202100045
Subject(s) - petri dish , stem cell , embryonic stem cell , stem cell niche , microbiology and biotechnology , materials science , biology , biomedical engineering , engineering , progenitor cell , biochemistry , genetics , gene
Abstract This article describes a technology to transform a petri dish into a microfluidic chip with a stem cell nest for stem cell niche engineering. A permanent magnet sphere is put in a 30 mm petri dish as an oscillator pump (O‐pump). An earphone‐sized actuator outside the petri dish receives the programmed audio signal from an MP3 player or a mobile phone to drive the in‐dish O‐pump for a microflow. There are guiding walls to form a wake area for the cell nest at the dish's center. The cell nest uses the wake to create a stable, ultraslow internal microcirculation flow. The retention half‐life of nanoparticles in the cell nest is 1419.8 ± 1.3 s. By setting the microfluidic program and supplementing five drops of the culture medium outside the cell nest every 2 d, the growth rate of embryonic stem cells in the cell nest during the 8 d automatic culture appears significantly optimized. The single clone area of embryonic stem cells increases from (3.57 ± 0.52) × 10 5 to (11.67 ± 1.33) × 10 5 µ m 2 after 2 d of the automatic culture and advances to (53.34 ± 8.37) × 10 5 µ m 2 after 4 d.