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A Versatile Dual‐Emission Fluorescent Microhybrid Enabling Visual Detection of Glucose and Other Oxidases‐Based Biocatalytic Systems
Author(s) -
Lu Xiaohui,
Wang Peipei,
Wang Yucong,
Liu Chenghui,
Li Zhengping
Publication year - 2016
Publication title -
advanced materials technologies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.184
H-Index - 42
ISSN - 2365-709X
DOI - 10.1002/admt.201600024
Subject(s) - glucose oxidase , fluorescence , naked eye , materials science , rhodamine , photochemistry , luminescence , cadmium telluride photovoltaics , nanotechnology , optoelectronics , chemistry , biosensor , optics , physics
A novel kind of dual‐emission microhybrids (DEMBs) is rationally fabricated which allows facile visual detection of glucose in serum. Here, green‐emitting rhodamine‐embedded silica microbeads (MBs) are used as the core of the DEMBs while a shell of CdTe quantum dots of red fluorescence is attached on the surface of MBs as the signal report unit. The red fluorescence of CdTe is susceptible to H 2 O 2 produced by the glucose/glucose oxidase (GOD) reaction while the inner green fluorescence of the MBs is inert. Therefore, with the assistance of GOD, increased glucose levels can lead to variations of the two fluorescence intensity ratios of DEMBs accompanied with continuous color changes from red to green under a UV lamp, which can be easily and distinctly identified by naked eye. This DEMB‐based sensing strategy can be directly used for assaying glucose in serum and the results agree well with those measured by commercial glucose meter, indicating that this glucose sensing system is reliable for practical applications. More importantly, since many oxidases‐based enzymatic reactions can all produce H 2 O 2 , the proposed design may serve as a generic platform for probing of H 2 O 2 ‐generating oxidases as well as their substrates.