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Sphere‐on‐Tube Biomimetic Hierarchical Nanostructures Coupled with Engineered Surfaces for Enhanced Photoelectrochemical Biosensing of Cancer Cells Expressing Folate Receptors
Author(s) -
Gao Ning,
Wang Xueying,
Feng Jinhui,
Li Xiaojian,
Wang Huan,
Fan Dawei,
Zhang Yong,
Ma Hongmin,
Wei Qin,
Ju Huangxian
Publication year - 2021
Publication title -
advanced materials interfaces
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.671
H-Index - 65
ISSN - 2196-7350
DOI - 10.1002/admi.202100421
Subject(s) - biosensor , materials science , folate receptor , cancer cell , nanostructure , nanotechnology , detection limit , receptor , polyethylene glycol , ligand (biochemistry) , biophysics , cancer , chemistry , biochemistry , biology , chromatography , genetics
The quantitative detection of cancer cells and the evaluation of folate receptor (FR) expression on cell membranes are of great significance for tumor‐related research. In this work, a photoelectrochemical (PEC) biosensing interface is proposed for detection of cancer cells based on the construction of nanostructured platforms coupled with surface engineering. A “sphere‐on‐tube” hierarchical nanostructure is fabricated by depositing biomimetic polydopamine nanospheres on TiO 2 nanotube arrays. Enhanced PEC responses are achieved with the formation of polyethylene glycol antifouling layers and the immobilization of folic acid as a recognition ligand. Based on the ligand–receptor interaction, the antifouling PEC biosensor exhibits a wide linear range for the detection of the breast cancer cell line MDA‐MB‐231 from 20 to 2.0 × 10 6 cells mL −1 with a low detection limit of 15 cells mL −1 (S/N = 3). The expressing differentiation of FR on several cancer cells is well discriminated. The proposed PEC cytosensing method shows great potential in detection of cancer cells expressing folate receptors.