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High‐Performance Flexible Bioelectrocatalysis Bioassay System Based on a Triphase Interface
Author(s) -
Cheng Qingqing,
Zhang Jun,
Wang Haili,
Wang Dandan,
Feng Xinjian,
Jiang Lei
Publication year - 2020
Publication title -
advanced materials interfaces
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.671
H-Index - 65
ISSN - 2196-7350
DOI - 10.1002/admi.201902172
Subject(s) - bioassay , analyte , biosensor , polydimethylsiloxane , materials science , nanotechnology , substrate (aquarium) , chromatography , chemistry , genetics , biology , oceanography , geology
Flexible biosensor technologies are essential for the development of noninvasive bioassays such as those based on sweat or interstitial fluid analysis. Cathodic measurement of oxidase catalytic product H 2 O 2 is an ideal principle for analyte detection, with O 2 serving as an electron mediator. Herein, the authors report a flexible bioassay system comprising a flexible superhydrophobic polydimethylsiloxane micropillar array substrate, a noble metal H 2 O 2 electrocatalyst‐decorated carbon nanotube film, and an oxidase enzyme top layer. The flexible bioassay system possesses a solid–liquid–air triphase bioelectrocatalysis reaction interface where oxygen levels are air phase dependent, hence constant and sufficient, which enhances and stabilizes the oxidase kinetics. In comparison with conventional flexible diphase bioassay systems, when used for analyte detection including but are not limited to glucose, choline, and lactate in body fluids, the triphase bioassay system displays a wide linear dynamic range and high selectivity. Moreover, the flexible bioassay system remains intact and fully operational after being bent over 500 times. Such a system should greatly promote the development of noninvasive biosensors for the monitoring of physiological biomarkers.