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Generation of Solvent‐Free 3D Lipid Structure Arrays on High Aspect Ratio Si Microwell Substrate
Author(s) -
Han Won Bae,
Kwak Rhokyun,
Kang Ji Yoon,
Kim Tae Song
Publication year - 2019
Publication title -
advanced materials interfaces
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.671
H-Index - 65
ISSN - 2196-7350
DOI - 10.1002/admi.201801554
Subject(s) - materials science , lipid bilayer , biosensor , nanotechnology , membrane , substrate (aquarium) , fluorescence , chemistry , biochemistry , physics , quantum mechanics , oceanography , geology
Artificial lipid membranes are versatile platforms that are used extensively in biological assays and sensing applications. Particularly, a 2D bilayer lipid membrane (BLM) has been focused on over the last several decades as it can be formed easily on solid supports by various methods. However, 3D lipid structures with structural advantages, such as large surface area that can accommodate a number of proteins and steric conformation that can react with target molecules efficiently, for use as highly sensitive sensors rarely have been studied due to the technical limitations of sealing and stability. Herein, the growth mechanism and condition for the formation of a solvent‐free, 3D lipid structure array (≈300 000 ea cm −2 ) tightly bound on a microwell array by a gentle hydration method are investigated. Then, applying an electric field during rehydration step, 3D unilamellar structure array with high uniformity (coefficient of variation (CV): ≈4.9%) can be formed. A pore‐forming protein assay (α‐hemolysin) shows that the proposed structures are completely sealed and have biofunctionality that allows them to be used for fluorescent signal measurement, which is essential for biosensing applications. This approach is expected to be a promising first step toward the development of artificial biomimetic sensory organs that have high sensitivity.