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A Novel AuNP‐Based Glucose Oxidase Mimic with Enhanced Activity and Selectivity Constructed by Molecular Imprinting and O 2 ‐Containing Nanoemulsion Embedding
Author(s) -
Fan Lin,
Lou Doudou,
Wu Haoan,
Zhang Xizhi,
Zhu Yefei,
Gu Ning,
Zhang Yu
Publication year - 2018
Publication title -
advanced materials interfaces
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.671
H-Index - 65
ISSN - 2196-7350
DOI - 10.1002/admi.201801070
Subject(s) - glucose oxidase , selectivity , catalysis , molecular imprinting , nanoparticle , materials science , bromide , combinatorial chemistry , colloidal gold , polymer , small molecule , biosensor , nanotechnology , chemistry , organic chemistry , biochemistry
In spite of the competitive advantages, inorganic nanoparticle mimic enzymes exhibit inherent disadvantages of limited catalytic efficiency and lacking selectivity. Here, a gold nanoparticle (AuNP)‐based mimic enzyme with significantly enhanced glucose selectivity and catalytic activity is constructed and demonstrated for the first time. Aminophenylboronic acid is employed to increase the affinity to glucose, as well as build molecular imprinted polymer shells to realize the selectivity for template molecules of glucose. Besides that, heptadecafluoro‐ n ‐octyl bromide nanoemulsion with the function of providing oxygen is introduced to gain a further improvement in catalytic activity, which successfully enhances the catalytic efficiency ( k cat / K m ) up to about 270‐fold. Based on the demonstrated catalytic properties, AuNP‐based glucose oxidase mimics are successfully applied in practical glucose detection of drinks and blood glucose.