Premium
Selective Cell Isolation by Transferrin Functionalized Silane–Carbon Soot Mediated Superhydrophobic Micropatterns
Author(s) -
Chate Govind P.,
Kale Narendra R.,
Khobragade Vrushali,
Rahane Chinmay,
Calderón Marcelo,
Banerjee Shashwat S.,
Khandare Jayant J.
Publication year - 2018
Publication title -
advanced materials interfaces
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.671
H-Index - 65
ISSN - 2196-7350
DOI - 10.1002/admi.201701581
Subject(s) - materials science , nanotechnology , silane , hela , adhesion , surface modification , cell adhesion , photothermal therapy , cell , wetting , chemistry , biochemistry , composite material
Surfaces that facilitate selective cell adhesion using specific targeting moieties have great implications in diagnostics, tissue engineering, and high throughput screenings. However, designing robust and spatially confined micropatterns for selective cell isolation on portable platform is highly challenging. Here, wettable, silane (Si) micropatterns holding covalently attached transferrin (Tf) for targeting Tf overexpressing cancer cells are reported. These micropatterns are separated by carbon soot based superhydrophobic regions that turn these targeting sites into surface tension confined “microwells.” These microwells facilitate capture of human colorectal carcinoma cells (HCT 116) and human cervical adenocarcinoma cells (HeLa) by confining their attachment to wettable region, thereby making isolation and spotting of the targeted cells more efficient. In addition, owing to its transparent trait, the Tf conjugated wettability based patterned chip offer real time optical monitoring of cell adhesion, cell growth, and cell behavior. The specific cell isolation using such surface has applications in devising cancer recurrence monitoring tests.