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Amplified Detection of a Unique Genomic Viral Marker Using Fluorescently Labeled Liposomes
Author(s) -
AbouShaheen Samir,
Fakih Hassan H.,
Kobeissi Jana M.,
Karam Pierre
Publication year - 2018
Publication title -
advanced materials interfaces
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.671
H-Index - 65
ISSN - 2196-7350
DOI - 10.1002/admi.201701527
Subject(s) - liposome , detection limit , oligonucleotide , fluorescence , flow cytometry , microbiology and biotechnology , materials science , chromatography , biology , chemistry , biochemistry , nanotechnology , dna , physics , quantum mechanics
Sensitive and enzyme‐free detection of specific oligonucleotide sequences is a pressing need in the field of precision and personalized medicine. In this work the detection of a unique genetic marker for hepatitis B virus with a low detection limit of 5 × 10 −12 m and a fast assay time is reported. In the assay presented here, fluorescently labeled liposomes are captured by gold‐decorated polystyrene beads using the target sequence as the building block. This is achieved by modifying the microbeads and the liposomes each with half of the complementary sequence. After a short incubation time with the target of interest, the fluorescent signal of the bridged bead–liposome assembly is detected via flow cytometry. Each liposome is labeled with ≈48 lipid soluble dyes resulting in an amplified fluorescent signal for every sensing event. The detection of the viral marker is even possible in an artificial serum‐spiked sample.