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Biofunctionalized Capillary Flow Channel Platform Integrated with 3D Nanostructured Matrix to Capture Circulating Tumor Cells
Author(s) -
Banerjee Shashwat S.,
Khutale Ganesh V.,
Khobragade Vrushali,
Kale Narendra R.,
Pore Milind,
Chate Govind P.,
JalotaBadhwar Archana,
Dongare Manoj,
Khandare Jayant J.
Publication year - 2017
Publication title -
advanced materials interfaces
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.671
H-Index - 65
ISSN - 2196-7350
DOI - 10.1002/admi.201600934
Subject(s) - circulating tumor cell , liquid biopsy , microchannel , materials science , cancer cell , metastasis , cancer research , cell sorting , cancer , biomedical engineering , metastatic breast cancer , nanotechnology , flow cytometry , breast cancer , biology , medicine , microbiology and biotechnology
Circulating tumor cells (CTCs) from peripheral blood account genetic information for cancer diagnosis and overall disease monitoring. Analysis of “liquid biopsy” holds immense promise as it may lead to new approaches for cancer treatment. The study reports effective and continuous flow microchannel system for isolating CTCs using transferrin conjugated 3D matrix synthesized by crosslinking polyethylene glycol‐Fe 3 O 4 nanostructures for rapid and efficient capturing of CTCs. The platform provides option of using multiple microchannel units in series that can influence higher cell‐capture efficiency due to increasing cell‐substrate contact frequency. CTCs are captured with high efficiency even at low concentration of target cells (~90% at 25 cells per mL blood). Furthermore, the study demonstrates that the cell‐capture performance is influenced by topographic interactions between nanostructure based matrix and cancer cells of interest. In addition, this study demonstrates the “proof of concept” using 3D microchannel system having capacity of simultaneously capturing and permanently eliminating CTCs from peripheral blood samples. Further, the study evaluates clinical samples of colon and breast cancer patients for rapid isolation of CTCs. Conclusively, the present platform demonstrates inordinate capacity for cancer cell sorting, biological studies of CTCs, and cancer metastasis, potentially benefiting the real time liquid biopsy and early prognosis of cancer.

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