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Capture and Release Erythrocyte from the Blood with Thermoresponsive and Core‐Sheath PCL/PNIPAAm Nanofibers
Author(s) -
Shi Qiang,
Hou Jianwen,
Xu Xiaodong,
Gao Jian,
Li Chunming,
Jin Jing,
Wong ShingChung,
Yin Jinghua
Publication year - 2016
Publication title -
advanced materials interfaces
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.671
H-Index - 65
ISSN - 2196-7350
DOI - 10.1002/admi.201500652
Subject(s) - nanofiber , polycaprolactone , materials science , electrospinning , adhesion , bovine serum albumin , chemical engineering , red blood cell , nanotechnology , polymer chemistry , polymer , chromatography , composite material , chemistry , biochemistry , engineering
A newly developed nanofiber platform for nonadherent cell capture and release is demonstrated. The nanofiber platform is fabricated by single‐spinneret electrospinning of bovine serum albumin (BSA)‐conjugated poly( N ‐isopropylacrylamide) (PNIPAAm) and polycaprolactone blends. The nanofibers possess core‐sheath structure with PNIPAAm as the sheath, which render the nanofibers switchable between hydrophilicity and hydrophobicity with the temperature change. As a result of BSA immobilization on the sheath part, the nanofibers resist platelet adhesion in the blood, facilitating the direct capture and isolation of red blood cells (RBCs) from the blood. Meanwhile, the captured RBCs are readily released from the nanofibers with the temperature stimuli. The capture and release efficiencies of up to 100% are achieved while maintaining cellular integrity and function. This work presents a promising platform to capture and release nonadherent cell effectively for subsequent molecular analysis and disease diagnosis.