Premium
Multimodal, Biomaterial‐Focused Anticoagulation via Superlow Fouling Zwitterionic Functional Groups Coupled with Anti‐Platelet Nitric Oxide Release
Author(s) -
Amoako Kagya A.,
Sundaram Harihara S.,
Suhaib Ahmed,
Jiang Shaoyi,
Cook Keith E.
Publication year - 2016
Publication title -
advanced materials interfaces
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.671
H-Index - 65
ISSN - 2196-7350
DOI - 10.1002/admi.201500646
Subject(s) - adsorption , platelet , materials science , fouling , coating , nitric oxide , biomaterial , polydimethylsiloxane , platelet activation , protein adsorption , chemical engineering , nuclear chemistry , chromatography , membrane , biomedical engineering , chemistry , composite material , nanotechnology , biochemistry , organic chemistry , medicine , immunology , engineering
The functions of anti‐fouling, zwitterionic polycarboxybetaine (pCB) coating, and anti‐platelet nitric oxide (NO) release replicate key anticoagulant properties of the endothelium. The two approaches, only tested separately thus far, are paired on gas permeable polydimethylsiloxane (PDMS) membranes and evaluated for fibrinogen (Fg) and platelet adsorption. Uncoated (control) and pCB‐coated PDMS separate sheep plasma (10 8 platelets per milliliter) and gas flow chambers within bioreactors used in this study, and either 100 or 0 ppm of NO/N 2 flows through the gas chamber so PDMS transfers NO into flowing plasma. Surface‐adsorbed platelets are quantified using a lactate dehydrogenase assay after 8h plasma recirculation. Fg and platelet adsorption on pCB‐coated PDMS are 10.40% ± 3.0% of control ( p < 0.01) and 23.3% ± 7.4% ( p < 0.01) of control, respectively. NO flux alone limits platelet adsorption to 79.0% ± 5.0% ( p < 0.05) of control. Together, NO and pCB reduce platelet adsorption to 6.90% ± 1.30% of control ( p < 0.001). The data suggest that pCB coating and NO act in concert to reduce platelet fouling at significantly higher levels than either pCB coating or NO release alone.