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Purification and Characterization of Protein Nanotubes Assembled from a Single Bacterial Microcompartment Shell Subunit
Author(s) -
Noël Christopher R.,
Cai Fei,
Kerfeld Cheryl A.
Publication year - 2016
Publication title -
advanced materials interfaces
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.671
H-Index - 65
ISSN - 2196-7350
DOI - 10.1002/admi.201500295
Subject(s) - mycobacterium smegmatis , escherichia coli , protein subunit , nanotechnology , biomaterial , computational biology , component (thermodynamics) , materials science , biology , biochemistry , physics , gene , mycobacterium tuberculosis , medicine , tuberculosis , pathology , thermodynamics
Bacterial microcompartment (BMC) nanotubes are isolated and characterized for the first time. RmmH, a component of the Mycobacterium smegmatis BMC shell, self‐assembles into protein nanotubes when expressed in Escherichia coli or by concentrating purified RmmH in vitro. BMC nanotubes offer a new biomaterial and spatial architecture that may be genetically or chemically modified and used as a molecular scaffold.