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High‐Resolution Imaging of Human Viruses in Liquid Droplets
Author(s) -
Jonaid GM,
Dearnaley William J.,
Casasanta Michael A.,
Kaylor Liam,
Berry Samantha,
Dukes Madeline J.,
Spilman Michael S.,
Gray Jennifer L.,
Kelly Deborah F.
Publication year - 2021
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.202103221
Subject(s) - materials science , nanotechnology , drug delivery , human health , temporal resolution , resolution (logic) , gene delivery , genetic enhancement , computer science , biology , optics , physics , gene , medicine , biochemistry , environmental health , artificial intelligence
Liquid‐phase electron microscopy (LP‐EM) is an exciting new area in the materials imaging field, providing unprecedented views of molecular processes. Time‐resolved insights from LP‐EM studies are a strong complement to the remarkable results achievable with other high‐resolution techniques. Here, the opportunities to expand LP‐EM technology beyond 2D temporal assessments and into the 3D regime are described. The results show new structures and dynamic insights of human viruses contained in minute volumes of liquid while acquired in a rapid timeframe. To develop this strategy, adeno‐associated virus (AAV) is used as a model system. AAV is a well‐known gene therapy vehicle with current applications involving drug delivery and vaccine development for COVID‐19. Improving the understanding of the physical properties of biological entities in a liquid state, as maintained in the human body, has broad societal implications for human health and disease.