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Production of Multiple Cell‐Laden Microtissue Spheroids with a Biomimetic Hepatic‐Lobule‐Like Structure
Author(s) -
Hong Gyusik,
Kim Jin,
Oh Hyeongkwon,
Yun Seokhwan,
Kim Chul Min,
Jeong YunMi,
Yun WonSoo,
Shim JinHyung,
Jang Ilho,
Kim CYoon,
Jin Songwan
Publication year - 2021
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.202102624
Subject(s) - spheroid , in vivo , microbiology and biotechnology , tissue engineering , cell , cell culture , regenerative medicine , biomedical engineering , microfluidics , in vitro , materials science , nanotechnology , stem cell , biology , medicine , biochemistry , genetics
The construction of an in vitro 3D cellular model to mimic the human liver is highly desired for drug discovery and clinical applications, such as patient‐specific treatment and cell‐based therapy in regenerative medicine. However, current bioprinting strategies are limited in their ability to generate multiple cell‐laden microtissues with biomimetic structures. This study presents a method for producing hepatic‐lobule‐like microtissue spheroids using a bioprinting system incorporating a precursor cartridge and microfluidic emulsification system. The multiple cell‐laden microtissue spheroids can be successfully generated at a speed of approximately 45 spheroids min −1 and with a uniform diameter. Hepatic and endothelial cells are patterned in a microtissue spheroid with the biomimetic structure of a liver lobule. The spheroids allow long‐term culture with high cell viability, and the structural integrity is maintained longer than that of non‐structured spheroids. Furthermore, structured spheroids show high MRP2, albumin, and CD31 expression levels. In addition, the in vivo study reveals that structured microtissue spheroids are stably engrafted. These results demonstrate that the method provides a valuable 3D structured microtissue spheroid model with lobule‐like constructs and liver functions.