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Live‐Cell Imaging: Time‐Gated FRET Nanoprobes for Autofluorescence‐Free Long‐Term In Vivo Imaging of Developing Zebrafish (Adv. Mater. 39/2020)
Author(s) -
Cardoso Dos Santos Marcelina,
Colin Ingrid,
Ribeiro Dos Santos Gabriel,
Susumu Kimihiro,
Demarque Michaël,
Medintz Igor L.,
Hildebrandt Niko
Publication year - 2020
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.202070291
Subject(s) - förster resonance energy transfer , autofluorescence , live cell imaging , fluorescence lifetime imaging microscopy , zebrafish , materials science , preclinical imaging , in vivo , fluorescence , nanotechnology , quantum dot , biophysics , microbiology and biotechnology , cell , chemistry , biology , optics , biochemistry , physics , gene
In article number 2003912, Marcelina Cardoso Dos Santos, Niko Hildebrandt, and co‐workers develop terbium‐to‐quantum‐dot fluorescence resonance energy transfer (FRET) nanohybrids for bright and autofluorescence‐free multiplexed imaging in live cells and long‐term imaging in developing zebrafish embryos over seven days, upon one‐cell stage microinjection. The work provides a strategy for advancing in vivo imaging applications beyond the capabilities of fluorescent proteins.