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Remote Control of Time‐Regulated Stretching of Ligand‐Presenting Nanocoils In Situ Regulates the Cyclic Adhesion and Differentiation of Stem Cells
Author(s) -
Min Sunhong,
Ko Min Jun,
Jung Hee Joon,
Kim Wonsik,
Han SeongBeom,
Kim Yuri,
Bae Gunhyu,
Lee Sungkyu,
Thangam Ramar,
Choi Hyojun,
Li Na,
Shin Jeong Eun,
Jeon Yoo Sang,
Park Hyeon Su,
Kim Yu Jin,
Sukumar Uday Kumar,
Song JaeJun,
Park SeungKeun,
Yu SeungHo,
Kang Yun Chan,
Lee KiBum,
Wei Qiang,
Kim DongHwee,
Han Seung Min,
Paulmurugan Ramasamy,
Kim Young Keun,
Kang Heemin
Publication year - 2021
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.202008353
Subject(s) - förster resonance energy transfer , ligand (biochemistry) , materials science , nanotechnology , adhesion , biophysics , cell adhesion , in vivo , integrin , nanostructure , cell , fluorescence , receptor , chemistry , biology , biochemistry , physics , microbiology and biotechnology , quantum mechanics , composite material
Native extracellular matrix (ECM) can exhibit cyclic nanoscale stretching and shrinking of ligands to regulate complex cell–material interactions. Designing materials that allow cyclic control of changes in intrinsic ligand‐presenting nanostructures in situ can emulate ECM dynamicity to regulate cellular adhesion. Unprecedented remote control of rapid, cyclic, and mechanical stretching (“ON”) and shrinking (“OFF”) of cell‐adhesive RGD ligand‐presenting magnetic nanocoils on a material surface in five repeated cycles are reported, thereby independently increasing and decreasing ligand pitch in nanocoils, respectively, without modulating ligand‐presenting surface area per nanocoil. It is demonstrated that cyclic switching “ON” (ligand nanostretching) facilitates time‐regulated integrin ligation, focal adhesion, spreading, YAP/TAZ mechanosensing, and differentiation of viable stem cells, both in vitro and in vivo. Fluorescence resonance energy transfer (FRET) imaging reveals magnetic switching “ON” (stretching) and “OFF” (shrinking) of the nanocoils inside animals. Versatile tuning of physical dimensions and elements of nanocoils by regulating electrodeposition conditions is also demonstrated. The study sheds novel insight into designing materials with connected ligand nanostructures that exhibit nanocoil‐specific nano‐spaced declustering, which is ineffective in nanowires, to facilitate cell adhesion. This unprecedented, independent, remote, and cytocompatible control of ligand nanopitch is promising for regulating the mechanosensing‐mediated differentiation of stem cells in vivo.