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In Vivo Three‐Photon Imaging of Lipids using Ultrabright Fluorogens with Aggregation‐Induced Emission
Author(s) -
Wang Shaowei,
Li Xueqi,
Chong Suet Yen,
Wang Xiaoyuan,
Chen Huan,
Chen Chengjian,
Ng Lai Guan,
Wang JiongWei,
Liu Bin
Publication year - 2021
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.202007490
Subject(s) - in vivo , fluorescence , aggregation induced emission , biophysics , preclinical imaging , materials science , fluorescence lifetime imaging microscopy , two photon excitation microscopy , nonalcoholic fatty liver disease , nanotechnology , pathology , fatty liver , biology , medicine , optics , disease , physics , microbiology and biotechnology
Fluorescent probes capable of in vivo lipids labeling are highly desirable for studying lipid‐accumulation‐related metabolic diseases, such as nonalcoholic fatty liver disease, type‐2 diabetes, and atherosclerosis. However, most of the current lipid‐specific fluorophores cannot be used for in vivo labeling due to their strong hydrophobicity. Herein, organic dots from bright luminogens with aggregation‐induced emission (AIEgen) are developed for in vivo labeling and three‐photon fluorescence imaging of lipid‐rich tissues, such as fatty liver, atherosclerotic plaques in brain vasculatures, and carotid arteries. The organic dots show excellent stability in an aqueous medium with high targeting specificity to lipids and strong three‐photon fluorescence in the far‐red/near‐infrared (NIR) region under NIR‐II laser excitation, which enables efficient in vivo labeling and imaging of lipids in deep tissues. The study will inspire the development of lipid‐targeting fluorophores for in vivo applications.