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A Bottom‐Up Approach to Red‐Emitting Molecular‐Based Nanoparticles with Natural Stealth Properties and their Use for Single‐Particle Tracking Deep in Brain Tissue
Author(s) -
Rosendale Morgane,
Flores Jessica,
Paviolo Chiara,
Pagano Paolo,
Daniel Jonathan,
Ferreira Joana,
Verlhac JeanBaptiste,
Groc Laurent,
Cognet Laurent,
BlanchardDesce Mireille
Publication year - 2021
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.202006644
Subject(s) - nanoparticle , nanotechnology , fluorescence , biofouling , membrane , biocompatibility , particle (ecology) , coating , aqueous solution , materials science , chemistry , chemical engineering , organic chemistry , optics , physics , biochemistry , oceanography , engineering , geology , metallurgy
Fluorescent nanoparticles dedicated to bioimaging applications should possess specific properties that have to be maintained in the aqueous, reactive, and crowded biological environment. These include chemical and photostability, small size (on the scale of subcellular structures), biocompatibility, high brightness, and good solubility. The latter is a major challenge for inorganic nanoparticles, which require surface coating to be made water soluble. Molecular‐based fluorescent organic nanoparticles (FONs) may prove a promising, spontaneously water‐soluble alternative, whose bottom‐up design allows for the fine‐tuning of individual properties. Here, the critical challenge of controlling the interaction of nanoparticles with cellular membranes is addressed. This is a report on bright, size‐tunable, red‐emitting, naturally stealthy FONs that do not require the use of antifouling agents to impede interactions with cellular membranes. As a proof of concept, single FONs diffusing up to 150 µm deep in brain tissue are imaged and tracked.